Automated, portable, low-cost bright-field and fluorescence microscope with autofocus and autoscanning capabilities.
نویسندگان
چکیده
Optical microscopy is a simple, yet essential, imaging technology. Conventional laboratory-grade optical microscopes are bulky and costly, confining their use to within laboratory settings and restricting their accessibility in regions of limited resources. With the aim of overcoming these limitations, we have realized a portable, low-cost, and highly automated optical microscope that integrates mass-manufactured components, including light-emitting diodes, a web camera, optical disk drives, and a microcontroller. Our implementation is capable of bright-field and fluorescence imaging with micrometer-scale resolution and controlled mechanical actuation of both the lens and sample. We interface the lighting, image capture, and mechanical actuators of the microscope into a single software environment, enabling automation of common microscope operations, such as image focusing and large-area sample visualization. Combination of mechanical actuation and software automation into a compact, low-cost microscope system is an important initial step toward the goal of making optical microscopy universally accessible, portable, and easy to use.
منابع مشابه
Portable, Battery-Operated, Low-Cost, Bright Field and Fluorescence Microscope
This study describes the design and evaluation of a portable bright-field and fluorescence microscope that can be manufactured for $240 USD. The microscope uses a battery-operated LED-based flashlight as the light source and achieves a resolution of 0.8 microm at 1000x magnification in fluorescence mode. We tested the diagnostic capability of this new instrument to identify infections caused by...
متن کاملContent-based Autofocusing in Automated Microscopy
Autofocusing is the fundamental step when it comes to image acquisition and analysis with automated microscopy devices. Despite all efforts that have been put into developing a reliable autofocus system, recent methods still lack robustness towards different microscope modes and distracting artefacts. This paper presents a novel automated focusing approach that is generally applicable to differ...
متن کاملA modular, open-source, slide-scanning microscope for diagnostic applications in resource-constrained settings
In this paper we report the development of a cost-effective, modular, open source, and fully automated slide-scanning microscope, composed entirely of easily available off-the-shelf parts, and capable of bright field and fluorescence modes. The automated X-Y stage is composed of two low-cost micrometer stages coupled to stepper motors operated in open-loop mode. The microscope is composed of a ...
متن کاملMultispot live-image autofocusing for high-throughput microscopy of fluorescently stained bacteria.
Screening by automated high-throughput microscopy has become a valuable research tool. An essential component of such systems is the autonomous acquisition of focused images. Here we describe the implementation of a high-precision autofocus routine for imaging of fluorescently stained bacteria on a commercially available microscope. We integrated various concepts and strategies that together su...
متن کاملFoldscope: Origami-Based Paper Microscope
Here we describe an ultra-low-cost origami-based approach for large-scale manufacturing of microscopes, specifically demonstrating brightfield, darkfield, and fluorescence microscopes. Merging principles of optical design with origami enables high-volume fabrication of microscopes from 2D media. Flexure mechanisms created via folding enable a flat compact design. Structural loops in folded pape...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied optics
دوره 51 14 شماره
صفحات -
تاریخ انتشار 2012